RATIONAL DESIGN OF SEQUENCE-SPECIFIC DNA LIGANDS FOR ARTIFICIAL CONTROL OF GENE-EXPRESSION

被引：8

作者：

HELENE, C

机构：

[1] Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, 75231 Paris Cedex 05, INSERM U.201, CNRS URA 481

来源：

PURE AND APPLIED CHEMISTRY | 1994年 / 66卷 / 04期

关键词：

D O I：

10.1351/pac199466040663

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oligonucleotides can be designed to bind to oligopurine oligopyrimidine sequences of double-helical DNA. Binding occurs in the major groove and a triple helix is formed. Bifunctional oligonucleotide-intercalator conjugates have an increased affinity due to intercalation either at the triplex-duplex junction or within the triple-helical structure. Some intercalating agents can be chemically or photochemidally activated to induce irreversible reactions at the target sequence. Oligonucleotide directed triple-helix formation can be used to control gene transcription. Chimeric oligonucleotides can also be designed to form a triple helix on a single-stranded target. These ''oligonucleotide clamps'' can block nucleic acid processing enzymes.

引用

页码：663 / 669

页数：7

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