RATIONAL DESIGN OF SEQUENCE-SPECIFIC DNA LIGANDS FOR ARTIFICIAL CONTROL OF GENE-EXPRESSION

被引：8

作者：

HELENE, C

机构：

[1] Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, 75231 Paris Cedex 05, INSERM U.201, CNRS URA 481

来源：

PURE AND APPLIED CHEMISTRY | 1994年 / 66卷 / 04期

关键词：

D O I：

10.1351/pac199466040663

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oligonucleotides can be designed to bind to oligopurine oligopyrimidine sequences of double-helical DNA. Binding occurs in the major groove and a triple helix is formed. Bifunctional oligonucleotide-intercalator conjugates have an increased affinity due to intercalation either at the triplex-duplex junction or within the triple-helical structure. Some intercalating agents can be chemically or photochemidally activated to induce irreversible reactions at the target sequence. Oligonucleotide directed triple-helix formation can be used to control gene transcription. Chimeric oligonucleotides can also be designed to form a triple helix on a single-stranded target. These ''oligonucleotide clamps'' can block nucleic acid processing enzymes.

引用

页码：663 / 669

页数：7

共 34 条

[31] SEQUENCE-SPECIFIC INTERCALATING AGENTS - INTERCALATION AT SPECIFIC SEQUENCES ON DUPLEX DNA VIA MAJOR GROOVE RECOGNITION BY OLIGONUCLEOTIDE INTERCALATOR CONJUGATES [J].

SUN, JS ;

FRANCOIS, JC ;

MONTENAYGARESTIER, T ;

SAISONBEHMOARAS, T ;

ROIG, V ;

THUONG, NT ;

HELENE, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (23) :9198-9202

[32]

SUN JS, 1991, CR ACAD SCI III-VIE, V313, P585

[33] SEQUENCE-SPECIFIC PHOTOINDUCED CROSS-LINKING OF THE 2 STRANDS OF DOUBLE-HELICAL DNA BY A PSORALEN COVALENTLY LINKED TO A TRIPLE HELIX-FORMING OLIGONUCLEOTIDE [J].

TAKASUGI, M ;

GUENDOUZ, A ;

CHASSIGNOL, M ;

DECOUT, JL ;

LHOMME, J ;

THUONG, NT ;

HELENE, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (13) :5602-5606

[34] SEQUENCE-SPECIFIC RECOGNITION AND MODIFICATION OF DOUBLE-HELICAL DNA BY OLIGONUCLEOTIDES [J].

THUONG, NT ;

HELENE, C .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1993, 32 (05) :666-690

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