TN5 MUTAGENESIS AND INSERTION REPLACEMENT IN AZOTOBACTER-VINELANDII

被引：4

作者：

CONTRERAS, A ^{[1
]}

MALDONADO, R ^{[1
]}

CASADESUS, J ^{[1
]}

机构：

[1] UNIV SEVILLA,DEPT GENET,APARTADO 1095,E-41080 SEVILLE,SPAIN

来源：

PLASMID | 1991年 / 25卷 / 01期

关键词：

D O I：

10.1016/0147-619X(91)90009-L

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Tn5 insertion mutants of Azotobacter vinelandii were isolated using vectors pJB4JI (IncP) and pGS9 (IncN). A procedure to replace Tn5 (Kmr) by its nontransposing derivative Tn5-131 (Tcr) was developed. For the replacement, a ColE1 derivative harboring Tn5-131 (pRZ131) was conjugally mobilized by the IncN plasmid pCU101 into A. vinelandii strains containing Tn5. Both plasmids are unable to be maintained in A. vinelandii, but the transient presence of pRZ131 allows recombination between the incoming and the resident Tn5 elements. Genetic and physical analysis showed that insertion replacements result in lower frequencies of Tn5-associated genomic rearrangements, thereby increasing the stability of Tn5-containing strains. © 1991.

引用

页码：76 / 80

页数：5

共 13 条

[11] CHARACTERIZATION OF AZOTOBACTER-VINELANDII DEOXYRIBONUCLEIC-ACID AND FOLDED CHROMOSOMES [J].

SADOFF, HL ;

SHIMEI, B ;

ELLIS, S .

JOURNAL OF BACTERIOLOGY, 1979, 138 (03) :871-877

[12] SUICIDE PLASMID VEHICLES FOR INSERTION MUTAGENESIS IN RHIZOBIUM-MELILOTI AND RELATED BACTERIA [J].

SELVARAJ, G ;

IYER, VN .

JOURNAL OF BACTERIOLOGY, 1983, 156 (03) :1292-1300

[13] REGULATION OF NITROGEN-METABOLISM IN AZOTOBACTER-VINELANDII - ISOLATION OF NTR AND GLNA GENES AND CONSTRUCTION OF NTR MUTANTS [J].

TOUKDARIAN, A ;

KENNEDY, C .

EMBO JOURNAL, 1986, 5 (02) :399-407

← 1 2 →