THE EFFECT OF ORGANIC MODIFIER IN THE MOBILE PHASE ON THE SEPARATION OF BILE-ACIDS AND ITS FLUORESCENT DERIVATIVES IN INCLUSION CHROMATOGRAPHY

被引：12

作者：

SHIMADA, K

YOSHIDA, H

KOMINE, Y

机构：

[1] Faculty of Pharmaceutical Sciences Kanazawa University, Kanazawa, 920

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 04期

关键词：

D O I：

10.1080/01483919108049274

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of organic modifier (methanol, acetonitrile, tetrahydrofuran) in the mobile phase on the separation of free bile acids and its fluorescent derivatives in reversed-phase high-performance liquid chromatography using cyclodextrin as a mobile phase additive was examined. Both methanol and acetonitrile were effective on the separation of free bile acids but the former was more effective than the latter on that of 3-(1-anthroyl)bile acids or bile acid 24-pyrenacyl esters. On the contrary tetrahydrofuran prevented the host-guest interaction.

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页码：605 / 617

页数：13

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