FLUOROMETRIC-DETERMINATION OF INDOMETHACIN IN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING IN-LINE OXIDATION WITH HYDROGEN-PEROXIDE

被引：4

作者：

KUBO, H

UMIGUCHI, Y

KINOSHITA, T

机构：

[1] School of Pharmaceutical Sciences, Kitasato university, Minato-ku., Tokyo 108

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 02期

关键词：

D O I：

10.1080/10826079308020924

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fluorometric method has been developed for the determination of indomethacin in serum by high performance liquid chromatography. indomethacin after deproteinization with acetonitrile was separated by reversed-phase chromatography with a neutral mobile phase (pH 7.0, 35% acetonitrile in phosphate buffer) containing hydrogen peroxide as a fluorogenic reagent, and was fluorometrically detected (Ex 358nm and Em 462nm) via in-line oxidation at high temperature (180-degrees-C). The calibration curve was linear over the range of 2.5-15.0mug/ml by injecting a volume of 20mul of deproteinized serum. The detection limit (signal-to-noise ratio-3) for indomethacin in serum was 0.5mug/ml using a 20-mul aliquot of deproteinized serum.

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页码：465 / 474

页数：10

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