THE RESPONSE OF BOVINE LYMPHOCYTES FROM LYMPH AND BLOOD TO PHYTOHEMAGGLUTININ

被引：12

作者：

BROWNLIE, J

STOTT, EJ

机构：

[1] Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury,, Berks. Berks.

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1979年 / 1卷 / 01期

关键词：

D O I：

10.1016/0165-2427(79)90003-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Optimal conditions for stimulation of bovine lymphocytes by Phytohaemagglutin (PHA) were established in a microtitre assay. Lymphocytes from efferent duct lymph responded vigorously, with specific incorporation of 100,000 to 200,000 cpm/well, providing the conditions of cell culture are carefully controlled. Peripheral blood lymphocytes responded over a wider range of cultural conditions but optimal specific incorporation was usually only 40-100,000 cpm/well. © 1979.

引用

页码：5 / 13

页数：9