ANALYSIS OF MAGNETIC-RESONANCE SPECTRA BY MOLE PERCENT - COMPARISON TO ABSOLUTE UNITS

被引:79
作者
KLUNK, WE [1 ]
XU, CJ [1 ]
PANCHALINGAM, K [1 ]
MCCLURE, RJ [1 ]
PETTEGREW, JW [1 ]
机构
[1] UNIV PITTSBURGH,SCH MED,WESTERN PSYCHIAT INST & CLIN,DEPT PSYCHIAT,NEUROPHYS LAB,PITTSBURGH,PA 15261
关键词
MOLE PERCENT; MAGNETIC RESONANCE SPECTROSCOPY; HPLC; PROTON; PHOSPHORUS; ALZHEIMERS DISEASE; PHOSPHODIESTERS; N-ACETYL-L-ASPARTATE; GLUTAMINE; GLUTAMATE; ASPARTATE; INOSITOL; GABA;
D O I
10.1016/0197-4580(94)90153-8
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
A variety of metabolites present in perchloric acid extracts of brain tissue were measured by (1)h and P-31 magnetic resonance spectroscopy (MRS) and HPLC in the same tissue sample and the MRS results were expressed both in terms of mole % and mu more/g based on an internal standard. The levels of 16 metabolites were compared by linear regression analysis and the mole % results were found to correlate very well with the results expressed as mu mole/g. To compare the two units under typical experimental conditions, the percent change in metabolites in a group of Alzheimer's disease brains was compared to a control group using both units. The results were essentially identical for the mole % and mu mole/g methods. We conclude that the use of the more % method of expressing MRS data yields results which are equivalent to those expressed in absolute units and suggest that, for in vivo MRS studies, use of the mole % method is preferable because fewer artifacts, such as partial Volume effects, are introduced.
引用
收藏
页码:133 / 140
页数:8
相关论文
共 22 条
  • [21] PROTON MAGNETIC-RESONANCE SPECTROSCOPY OF HUMAN BRAIN - APPLICATIONS TO NORMAL WHITE MATTER, CHRONIC INFARCTION, AND MRI WHITE MATTER SIGNAL HYPERINTENSITIES
    SAPPEYMARINIER, D
    CALABRESE, G
    HETHERINGTON, HP
    FISHER, SNG
    DEICKEN, R
    VANDYKE, C
    FEIN, G
    WEINER, MW
    [J]. MAGNETIC RESONANCE IN MEDICINE, 1992, 26 (02) : 313 - 327
  • [22] WOESSNER DE, 1989, NMR PRINCIPLES APPLI, P37