Cyclocolorenone, a sesquiterpene ketone of the aromadendrane class, was isolated from Magnolia grandiflora L. by bioassay-directed fractionation and its identity confirmed by UV, IR, NMR, and MS techniques. It showed good growth-inhibitory activity in an etiolated wheat coleoptile assay. Cyclocolorenone was also phytotoxic to greenhouse-grown corn, bean, and tobacco plants and displayed antimicrobial activity.