HUMAN RIBOSOMAL-RNA GENE REPEATS ARE LOCALIZED IN THE DENSE FIBRILLAR COMPONENT OF NUCLEOLI - LIGHT AND ELECTRON-MICROSCOPIC INSITU HYBRIDIZATION IN HUMAN SERTOLI CELLS

被引：75

作者：

WACHTLER, F

SCHOFER, C

MOSGOLLER, W

WEIPOLTSHAMMER, K

SCHWARZACHER, HG

GUICHAOUA, M

HARTUNG, M

STAHL, A

BERGELEFRANC, JL

GONZALEZ, I

SYLVESTER, J

机构：

[1] FAC MED MARSEILLE,BIOCHEM LAB,F-13385 MARSEILLE 5,FRANCE

[2] FAC MED MARSEILLE,GENET LAB,F-13385 MARSEILLE 5,FRANCE

[3] HAHNEMANN UNIV,DEPT PATHOL,PHILADELPHIA,PA 19102

来源：

EXPERIMENTAL CELL RESEARCH | 1992年 / 198卷 / 01期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1016/0014-4827(92)90159-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The distribution of the human ribosomal gene repeat within human Sertoli cell nucleoli was investigated with the help of DNA-DNA in situ hybridization at the light and electron microscopic level. Probes from both the transcribed part of the gene repeat and the "non-transcribed" spacer were found to hybridize predominantly to the dense fibrillar component of nucleoli. It therefore can be concluded that the dense fibrillar component of nucleoli is the major site of the intranucleolar location of the ribosomal DNA. This holds true not only for the dense fibrillar component adjacent to fibrillar centers, but also for the dense fibrillar component remote from the fibrillar centers. © 1992.

引用

页码：135 / 143

页数：9