NOVEL SINGLE STANDARD CALIBRATION AND DILUTION METHOD PERFORMED BY THE SEQUENTIAL INJECTION TECHNIQUE

A novel single standard calibration and dilution method utilizing the sequential injection analysis (SIA) technique is described. The SIA manifold employs a dilution conduit for storing a concentration gradient of an injected analyte, which provides for a variable calibration and dilution scheme suitable for both single and multizone analyses by taking selected aliquots from the gradient. This paper describes the principles of the method, the experimental characterization of the SIA manifold with Bromothymol Blue dye using a spectrophotometric detector and the application of the method to glucose determination using glucose oxidase by sensor injection analysis and by multizone stopped-flow analysis.