17-BETA-ESTRADIOL MIMICS LIGAND ACTIVITY OF THE C-ERBB2 PROTOONCOGENE PRODUCT

被引：95

作者：

MATSUDA, S ^{[1
]}

KADOWAKI, Y ^{[1
]}

ICHINO, M ^{[1
]}

AKIYAMA, T ^{[1
]}

TOYOSHIMA, K ^{[1
]}

YAMAMOTO, T ^{[1
]}

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,SUITA,OSAKA 565,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 22期

关键词：

PROTEIN-TYROSINE PHOSPHORYLATION; GROWTH FACTOR RECEPTOR; STEROID HORMONE;

D O I：

10.1073/pnas.90.22.10803

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report here the physical and functional interaction of estrogen with the ErbB2 protein p185c-erbB2. The ErbB2 protein immunoprecipitated from estrogen-treated [10(-8) to 10(-6) M 17beta-estradiol (E2)] RC cells showed higher autophosphorylation activity than that from untreated cells. Likewise autophosphorylation activity of ErbB2 protein from untreated cells was stimulated in vitro by E2. In addition, E2 treatment induced down-regulation of ErbB2 protein from the detergent-soluble fraction of the RC cells within 15 min. E2 also induced morphological transformation of the RC cells but not of the parental NIH 3T3 cells, which express little c-erbB2 under the same experimental conditions. This morphological transformation of RC cells was reversed by tamoxifen. However, E2 treatment did not induce anchorage-independent growth of RC cells. Scatchard analysis revealed E2 binding to the ErbB2 protein on RC cells; the K(d) value was 2.7 nM. E2 did not bind appreciably to the parental NIH 3T3 cells or cells expressing an ErbB2 protein lacking most of its extracellular domain. These data suggest that estrogen plays an important role in ErbB2-mediated signaling.

引用

页码：10803 / 10807

页数：5

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