RAPID AND TRANSIENT EXPRESSION OF ETS2 IN MATURE MACROPHAGES FOLLOWING STIMULATION WITH CMGF, LPS, AND PKC ACTIVATORS

被引:57
作者
BOULUKOS, KE
POGNONEC, P
SARIBAN, E
BAILLY, M
LAGROU, C
GHYSDAEL, J
机构
[1] INST PASTEUR,CNRS,UA 041160,INSERM,U186,F-59019 LILLE,FRANCE
[2] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,CLIN PHARMACOL LAB,BOSTON,MA 02115
关键词
c-ets-2; cMGF; Early response genes; Macrophages; PKC; Proto-oncogene;
D O I
10.1101/gad.4.3.401
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We reported previously that Ets2 is expressed in normal and transformed macrophages. We show here that the expression of both c-ets-2 mRNA and proteins is induced rapidly and transiently in chicken nondividing bone marrow-derived macrophages but not in E26-transformed myeloblasts in response to chicken myelomonocytic growth factor (cMGF), and avian hematopoietic growth factor required for survival, proliferation, and colony formation of avian myeloid cells. c-ets-2 expression is also rapidly induced in chicken bone marrow-derived macrophages, human monocytes, and mouse peritoneal macrophages in response to LPS and/or PKC activators. The rapid induction of Ets2 after treatment of chicken bone marrow-derived macrophages by cMGF is blunted after down-regulation or inactivation of PKC, suggesting a role of PKC in the cMGF-induced signal transduction pathway. Because Ets2 is localized in the nucleus of macrophages and binds to DNA in vitro, the kinetics of its expression suggest a role for Ets2 in the transduction within the nucleus of specific signals received at the cell membrane and involved in securing the survival and/or the development of functional competence of these cells.
引用
收藏
页码:401 / 409
页数:9
相关论文
共 44 条
[41]  
TANNENBAUM CS, 1988, J IMMUNOL, V140, P3640
[42]  
TODD RF, 1985, J IMMUNOL, V135, P3869
[43]   CONCOMITANT SECRETION OF PROUROKINASE AND OF A PLASMINOGEN-ACTIVATOR SPECIFIC INHIBITOR BY CULTURED HUMAN MONOCYTES-MACROPHAGES [J].
VASSALLI, JD ;
DAYER, JM ;
WOHLWEND, A ;
BELIN, D .
JOURNAL OF EXPERIMENTAL MEDICINE, 1984, 159 (06) :1653-1668
[44]   THE ETS SEQUENCE FROM THE TRANSFORMING GENE OF AVIAN ERYTHROBLASTOSIS VIRUS, E26, HAS UNIQUE DOMAINS ON HUMAN CHROMOSOME-11 AND CHROMOSOME-21 - BOTH LOCI ARE TRANSCRIPTIONALLY ACTIVE [J].
WATSON, DK ;
MCWILLIAMSSMITH, MJ ;
NUNN, MF ;
DUESBERG, PH ;
OBRIEN, SJ ;
PAPAS, TS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (21) :7294-7298