STEROL SIDE-CHAIN CLEAVAGE WITH IMMOBILIZED MYCOBACTERIUM CELLS IN WATER-IMMISCIBLE ORGANIC-SOLVENTS

被引：50

作者：

DIAS, ACP ^{[1
]}

CABRAL, JMS ^{[1
]}

PINHEIRO, HM ^{[1
]}

机构：

[1] INST SUPER TECN,DEPT ENGN QUIM,ENGN BIOQUIM LAB,LISBON,PORTUGAL

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1994年 / 16卷 / 08期

关键词：

STEROL; SIDE-CHAIN DEGRADATION; MYCOBACTERIUM SP; IMMOBILIZED CELLS; ORGANIC SOLVENTS;

D O I：

10.1016/0141-0229(94)90094-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The possibility of using whole Mycobacterium sp. cells for the selective degradation of the side-chain of sitosterol in an organic bioconversion medium was investigated. Sterol solubility limits wets estimated and free-cell biocompatibility tests were carried out, using a range of water-immiscible solvents. Among these, phthalates exhibited good biocompatibility and sterol-solubilizing capacities. Several organic and inorganic matrices were tested for the immobilization of Mycobacterium sp. cells by surface adhesion. Celite led to the best results, being thus selected for beta-sitosterol side-chain degradation tests in phthalates and in aqueous medium. Cells entrapped in kappa-carrageenan and in polyurethane foams were also used in these tests. The highest degradation activities were obtained with cells immobilized in Celite, with bis(2-ethylhexyl)phthalate as the conversion medium, resulting in molar conversion yields up to 70%, with respect to beta-sitosterol (5 gl(-1)). Further activity and stability tests revealed that this bioconversion system is markedly temperature dependent.

引用

页码：708 / 714

页数：7

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