CYCLIC-GMP IN THE PERFUSED RAT-HEART - EFFECT OF ISCHEMIA, ANOXIA AND NITRIC-OXIDE SYNTHASE INHIBITOR

被引：50

作者：

DEPRE, C ^{[1
]}

HUE, L ^{[1
]}

机构：

[1] UNIV CATHOLIQUE LOUVAIN, SCH MED, INT INST CELLULAR & MOLEC PATHOL, HORMONE & METAB RES UNIT, B-1200 BRUSSELS, BELGIUM

来源：

FEBS LETTERS | 1994年 / 345卷 / 2-3期

关键词：

CYCLIC AMP; CYCLIC GMP; ISCHEMIA; GLYCOGEN; HEART; NITRIC OXIDE;

D O I：

10.1016/0014-5793(94)00459-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Working rat hearts perfused with 5.5 mM glucose were submitted to a 10-min period of no-flow ischaemia or anoxia. Both conditions stimulated glycogenolysis, activated phosphorylase and increased cyclic GMP content, although the time course of these changes differed in anoxia and ischaemia. Changes in cyclic GMP content were not correlated with glycogenolysis or phosphorylase activation. Perfusion with 1 mu M L-nitroarginine methylester, an inhibitor of nitric oxide synthase, decreased cGMP concentration under normoxic conditions and abolished the ischaemia-induced increase in cGMP. The inhibitor decreased the coronary flow without affecting the overall working performance of the hearts under normoxic conditions.

引用

页码：241 / 245

页数：5

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