POSTTRANSCRIPTIONAL REGULATION OF THE HETEROCHRONIC GENE LIN-14 BY LIN-4 MEDIATES TEMPORAL PATTERN-FORMATION IN C-ELEGANS

被引:3104
作者
WIGHTMAN, B [1 ]
HA, I [1 ]
RUVKUN, G [1 ]
机构
[1] MASSACHUSETTS GEN HOSP, DEPT MOLEC BIOL, BOSTON, MA 02114 USA
关键词
D O I
10.1016/0092-8674(93)90530-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During C. elegans development, the temporal pattern of many cell lineages is specified by graded activity of the heterochronic gene Lin-14. Here we demonstrate that a temporal gradient in Lin-14 protein is generated posttranscriptionally by multiple elements in the lin-14 3'UTR that are regulated by the heterochronic gene Lin-4. The lin-14 3'UTR is both necessary and sufficient to confer lin-4-mediated posttranscriptional temporal regulation. The function of the lin-14 3'UTR is conserved between C. elegans and C. briggsae. Among the conserved sequences are seven elements that are each complementary to the lin-4 RNAs. A reporter gene bearing three of these elements shows partial temporal gradient activity. These data suggest a molecular mechanism for Lin-14p temporal gradient formation: the lin-4 RNAs base pair to sites in the lin-14 3'UTR to form multiple RNA duplexes that down-regulate lin-14 translation.
引用
收藏
页码:855 / 862
页数:8
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