IDENTIFICATION OF CHICKEN CALBINDIN-D28K PRE-MESSENGER-RNA SEQUENCES BY POLYMERASE CHAIN-REACTION

被引:16
作者
FERRARI, S
BATTINI, R
机构
[1] Istituto di Chimica Biologica, Università di Modena, 41000 Modena
关键词
D O I
10.1016/0006-291X(90)92339-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A transcribed RNA sequence encompassing the junction between the first intron and the second exon of the chicken calbindin D28K gene was copied in a cDNA fragment and subsequently amplified by polymerase chain reaction. When intestinal RNA is used as template, the appearance of the 161 bp amplified fragment is strictly dependent on the vitamin D status of the animal. In fact no amplified fragment is obtained when the RNA is extracted from the intestine of vitamin D-deficient chickens, while it is easily detected when the RNA is extracted only 30 min after injection with 1,25-dihydroxycholecalciferol. Conversely, the amplified fragment is obtained, irrespectively of the vitamin D status of the animal, when the RNA template is extracted from the brain. The appearance of unspliced RNA sequences upon vitamin D induction is followed, after a 30 min lag, by the appearance of the corresponding mature mRNA sequences. © 1990.
引用
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页码:430 / 436
页数:7
相关论文
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