PHOTOCHEMICAL RELEASE OF PROTECTED OLIGONUCLEOTIDES CONTAINING 3'-GLYCOLATE TERMINI

被引：10

作者：

GREENBERG, MM

机构：

[1] Department of Chemistry, Colorado State University Fort Collins

来源：

TETRAHEDRON | 1995年 / 51卷 / 01期

关键词：

D O I：

10.1016/0040-4020(94)00946-R

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Protected oligonucleotides containing 3'-glycolate termini that are suitable for further elaboration are released from their solid phase supports using the o-nitrobenzyl intramolecular photochemical redox reaction. The yields of isolated oligonucleotides of varying sequence, ranging between 12 and 20 nucleotides long are between 45% and 79%, under conditions that have been previously shown to form less than 2% thymine.thymine photodimers.

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页码：29 / 38

页数：10

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