STIMULATION OF GROWTH OF HUMAN BREAST-CANCER CELLS (T47D) BY PLATELET DERIVED GROWTH-FACTOR

被引：19

作者：

GINSBURG, E ^{[1
]}

VONDERHAAR, BK ^{[1
]}

机构：

[1] NCI, TUMOR IMMUNOL & BIOL LAB, BLDG 10, ROOM 5B56, BETHESDA, MD 20892 USA

来源：

CANCER LETTERS | 1991年 / 58卷 / 1-2期

关键词：

BREAST CELLS; T47D; PLATELET DERIVED GROWTH FACTOR; MCF-7;

D O I：

10.1016/0304-3835(91)90036-H

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The human breast cancer cell lines T47D and MCF-7, respond to the mitogenic action of exogenously added PDGF with a 2 - 3-fold increase in cell number. The maximal response was obtained at a concentration of 1.25 half maximal units/ml (125 ng/ml). PDGF-AA was even more effective than PDGF-AB while PDGF-BB was without effect. The growth-enhancing activity of PDGF was completely abolished by Fab fragments of anti PDGF. Within 7 min of addition of PDGF to cultures of T47D cells, specific phosphorylation of a 65-kDa protein was observed. T47D cells contain specific receptors for PDGF with approximately 4-7 x 10(4) sites (kDa of 3-4 x 10(-10) M) per cell.

引用

页码：137 / 144

页数：8

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