2 HIGH MOLECULAR MASS PROTEASES FROM SEA-URCHIN SPERM

被引：16

作者：

INABA, K

AKAZOME, Y

MORISAWA, M

机构：

[1] Misaki Marine Biological Station, Faculty of Science, University of Tokyo, Miura, Kanagawa

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91784-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-types of high molecular mass proteases have been purified from sea urchin sperm using DEAE-Sephacel, hydroxylapatite and Superdex 200 column chromatography. Both proteases showed similar hydrolyzing activities toward synthetic peptides, but they differed in the molecular mass and peptide composition. One was probably identical to a proteasome (multicatalytic proteinase), judging from its molecular mass (650 kDa) and polypeptide composition. The other one was composed of several polypeptides with molecular masses ranging from 24 kDa to 125 kDa and its molecular mass was estimated as 950 kDa by gel filtration. These two proteases, however, were closely related to each other. Immunological studies revealed that the 950-kDa protease comprised at least five subunits of the 650-kDa protease. © 1992.

引用

页码：667 / 674

页数：8

共 16 条

[1]

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