DEVELOPMENT AND EVALUATION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR PLASMODIUM-VIVAX-VK247 SPOROZOITES

被引：103

作者：

WIRTZ, RA ^{[1
]}

SATTABONGKOT, J ^{[1
]}

HALL, T ^{[1
]}

BURKOT, TR ^{[1
]}

ROSENBERG, R ^{[1
]}

机构：

[1] WALTER REED ARMY MED CTR,DEPT IMMUNOL,DIV COMMUNICABLE DIS & IMMUNOL,WASHINGTON,DC 20307

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 1992年 / 29卷 / 05期

关键词：

PLASMODIUM-VIVAX; CIRCUMSPOROZOITE PROTEIN DETECTION; ELISA;

D O I：

10.1093/jmedent/29.5.854

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

An enzyme-linked immunosorbent assay (ELISA) for the Plasmodium vivax-VK247 (variant) circumsporozoite (CS) protein was developed and evaluated using sporozoites produced by feeding mosquitoes on Thai patients with parasitologically confirmed P. vivax infections. The ELISA had a detection threshold of fewer than 50 sporozoites. Using this assay in conjunction with an ELISA for the VK210 polymorph, nearly 16% of the 235 P. vivax cases produced sporozoites positive only for the variant; 69% produced sporozoites positive only in the VK210 assay; and 15% were positive in both assays, indicating mixed infections. Twelve cases (5%) produced sporozoites negative in one assay and with unexpectedly low activity in the other ELISA, indicating the possibility of other CS protein polymorphs.

引用

页码：854 / 857

页数：4

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