REGULATORY ROLE OF A NEUROTRANSMITTER (5-HT) ON GLIAL NA+/K+-ATPASE IN THE RAT-BRAIN

被引：21

作者：

MERCADO, R ^{[1
]}

HERNANDEZ, J ^{[1
]}

机构：

[1] INST POLITECN NACL,CTR INVEST ESTUD,DEPT PHYSIOL BIOPHYS & NEUROSCI,NEUROTOGENY LAB,MEXICO CITY 07000,DF,MEXICO

来源：

NEUROCHEMISTRY INTERNATIONAL | 1992年 / 21卷 / 01期

关键词：

D O I：

10.1016/0197-0186(92)90074-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present work we studied the effect of serotonin (5-HT) on the kinetics of Na+/K+-ATPase in subcellular preparations of the cerebral cortex from male Wistar rats using various concentrations of ATP and K+ with and without added 5-HT. Also we studied the effect of 5-HT on the enzyme in glial or neuronal preparations. The results indicated that there was a significant increase (P < 0.05) of the V(max) in the presence of 5-HT in the whole tissue preparation (homogenate) but not in the subcellular fractions, suggesting that the interaction could be preferentially with the glial pump. Further results supported that this was the case since activation by 5-HT was mainly in the glial preparations. Kinetic data and the binding of [H-3]ouabain supported that the enzyme is activated by 5-HT through the exposure of more enzymatic active sites.

引用

页码：119 / 127

页数：9

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