OBSERVATION OF MICROORGANISM COLONIES USING A SCANNING-LASER-BEAM PH-SENSING MICROSCOPE

被引：44

作者：

NAKAO, M ^{[1
]}

INOUE, S ^{[1
]}

OISHI, R ^{[1
]}

YOSHINOBU, T ^{[1
]}

IWASAKI, H ^{[1
]}

机构：

[1] NAKANO VINEGAR CO LTD,NAKANO CENT RES INST,HANDA,AICHI 475,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 79卷 / 02期

关键词：

PH SENSOR; PH-IMAGING SENSOR; MICROORGANISM; METABOLISM; COLONY-COUNTING;

D O I：

10.1016/0922-338X(95)94085-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results.

引用

页码：163 / 166

页数：4

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