MULTIMYCOTOXIN DETECTION AND CLEANUP METHOD FOR AFLATOXINS, OCHRATOXIN AND ZEARALENONE IN ANIMAL FEED INGREDIENTS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND GEL-PERMEATION CHROMATOGRAPHY

被引：23

作者：

DUNNE, C

MEANEY, M

SMYTH, M

TUINSTRA, LGMT

机构：

[1] STATE INST QUAL CONTROL AGR PROD,BORNSESTEEG 45,6708 PD WAGENINGEN,NETHERLANDS

[2] DUBLIN CITY UNIV,SCH CHEM SCI,DUBLIN 9,IRELAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 629卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)87036-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and reliable method is described for the determination of aflatoxins B1, B2, G1 and G2, ochratoxin A and zearalenone in animal feed ingredients. A multi-toxin extraction and clean-up procedure is used, with dichloromethane-1 M hydrochloric acid (10:1) being used for the extraction and gel permeation chromatography being used for the clean-up. The liquid chromatographic method developed for the separation of the six mycotoxins involves gradient elution with a reversed-phase C18 column and fluorescence detection. Recoveries, repeatability and reproducibility have been determined on maize, palm and wheat. The detection limits varied depending on the type of feed.

引用

页码：229 / 235

页数：7

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