MICELLAR ELECTROKINETIC CHROMATOGRAPHY OF HYDROXYPROLINE AND OTHER SECONDARY AMINO-ACIDS IN BIOLOGICAL SAMPLES WITH LASER-INDUCED FLUORESCENCE DETECTION

被引：26

作者：

CHAN, KC

JANINI, GM

MUSCHIK, GM

ISSAQ, HJ

机构：

[1] PRI/DynCorp, NCI Frederick Cancer Research and Development Center, Frederick, MD 21702, P.O. Box B

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 622卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80276-A

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

Micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) was used for the rapid and sensitive detection of hydroxyproline in serum and hydrolyzed urine that were pre-column derivatized with 9-fluorenylmethyl chloroformate (FMOC). The application of the combined o-phthalaldehyde (OPA)/FMOC derivatization in MEKC for the selective detection of secondary amino acids in biological samples is investigated.

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页码：269 / 273

页数：5

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