RAPID AND SIMPLE METHOD FOR THE DETERMINATION OF NITRITE IN SYNAPTOSOMAL SUPERFUSATES BY LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：8

作者：

GORBUNOV, N ^{[1
]}

ESPOSITO, E ^{[1
]}

机构：

[1] IST RIC FARMACOL MARIO NEGRI,CONSORZIO MARIO NEGRI SUD,I-66030 SANTA MARIA IMBAT,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 619卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80456-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and simple technique using reversed-phase high-performance liquid chromatography with electrochemical detection (HPLC-ED) was developed for the determination of nitrite. Standard solutions of sodium nitrite produced a linear current response over the concentration range 1.5-30 muM. The chromatographic peaks of sodium nitrite were superimposable with peaks obtained after injection into the chromatograph of supernatants from rat cerebellar synaptosomes. The concentrations of nitrite in synaptosomes measured with HPLC-ED were similar to those found with a widely used colorimetric procedure. Therefore, this new method may represent a useful means of measuring nitrite in biological samples.

引用

页码：133 / 136

页数：4

共 16 条

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