CLEAVAGE MAP OF THE TOL PLASMID OF PSEUDOMONAS-PUTIDA MT-2

被引：40

作者：

DOWNING, R

BRODA, P

机构：

[1] Department of Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR, Kings Buildings

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 177卷 / 01期

关键词：

D O I：

10.1007/BF00267270

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants. © 1979 Springer-Verlag.

引用

页码：189 / 191

页数：3

共 8 条

[1] 2 MODES OF LOSS OF TOL FUNCTION FROM PSEUDOMONAS-PUTIDA MT-2
BAYLEY, SA
DUGGLEBY, CJ
WORSEY, MJ
WILLIAMS, PA
HARDY, KG
BRODA, P
[J]. MOLECULAR & GENERAL GENETICS, 1977, 154 (02): : 203 - 204
[2] TOL IS A BROAD-HOST-RANGE PLASMID
BENSON, S
SHAPIRO, J
[J]. JOURNAL OF BACTERIOLOGY, 1978, 135 (01) : 278 - 280
[3] TRANSPOSITION OF PLASMID DNA SEGMENTS SPECIFYING HYDROCARBON DEGRADATION AND THEIR EXPRESSION IN VARIOUS MICROORGANISMS
CHAKRABARTY, AM
FRIELLO, DA
BOPP, LH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (07) : 3109 - 3112
[4] RESTRICTION ENDONUCLEASE MAPPING OF A PLASMID THAT CONFERS ONCOGENICITY UPON AGROBACTERIUM-TUMEFACIENS STRAIN B6-806
CHILTON, MD
MONTOYA, AL
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DRUMMOND, MH
NUTTER, R
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NESTER, EW
[J]. PLASMID, 1978, 1 (02) : 254 - 269
[5] DNA-DNA HYBRIDIZATION ON MEMBRANE FILTERS - CONVENIENT METHOD USING FORMAMIDE
DAWID, IB
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 477 (02) : 191 - 194
[6] ENDONUCLEASE CLEAVAGE MAP OF THE PLASMID PWWO-8, A DERIVATIVE OF THE TOL PLASMID OF PSEUDOMONAS-PUTIDA MT-2
DOWNING, RG
DUGGLEBY, CJ
VILLEMS, R
BRODA, P
[J]. MOLECULAR & GENERAL GENETICS, 1979, 168 (01): : 97 - 99
[7] TRANSPOSITION OF PSEUDOMONAS TOLUENE-DEGRADING GENES AND EXPRESSION IN ESCHERICHIA-COLI
JACOBY, GA
ROGERS, JE
JACOB, AE
HEDGES, RW
[J]. NATURE, 1978, 274 (5667) : 179 - 180
[8] ISOLATION OF TOL AND RP4 RECOMBINANTS BY INTEGRATIVE SUPPRESSION
NAKAZAWA, T
HAYASHI, E
YOKOTA, T
EBINA, Y
NAKAZAWA, A
[J]. JOURNAL OF BACTERIOLOGY, 1978, 134 (01) : 270 - 277

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