ELECTROPHORETIC KARYOTYPE AND GENE ASSIGNMENT TO RESOLVED CHROMOSOMES OF TRICHODERMA SPP

被引：21

作者：

HERRERAESTRELLA, A ^{[1
]}

GOLDMAN, GH ^{[1
]}

VANMONTAGU, M ^{[1
]}

GEREMIA, RA ^{[1
]}

机构：

[1] STATE UNIV GHENT,GENET LAB,LEDEGANCKSTR 35,B-9000 GHENT,BELGIUM

来源：

MOLECULAR MICROBIOLOGY | 1993年 / 7卷 / 04期

关键词：

D O I：

10.1111/j.1365-2958.1993.tb01142.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A molecular karyotype for three different Trichoderma species (T. harzianum, T. viride, and T. reesei) was determined by using two different systems: countour-clamped electric-field and rotating-electrode electrophoresis. Six chromosomal DNA bands were observed in T. harzianum and T. reesei and five in T. viride. The sizes of these molecules were estimated by their mobility relative to the Schizosaccharomyces pombe chromosomes and ranged between 2.2 and 7.4 megabase pairs (mbp). The estimated genome sizes range f rom 31 to 39 mbp. A number of genes were located in the different chromosomes by means of Southern analysis. The implications of these findings are discussed.

引用

页码：515 / 521

页数：7

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