HETEROGENEITY OF THE AMINO-ACID-SEQUENCES OF ESCHERICHIA-COLI SHIGA-LIKE TOXIN TYPE-I OPERONS

被引：73

作者：

PATON, AW

BEUTIN, L

PATON, JC

机构：

[1] WOMENS & CHILDRENS HOSP,DEPT MICROBIOL,ADELAIDE,SA 5006,AUSTRALIA

[2] ROBERT KOCH INST,DEPT MICROBIOL,E COLI REFERENCE LAB,D-13353 BERLIN,GERMANY

来源：

GENE | 1995年 / 153卷 / 01期

关键词：

PATHOGENESIS; SHIGA TOXIN; VEROTOXIN;

D O I：

10.1016/0378-1119(94)00777-P

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

PCR was used to amplify approx. 1470-bp segments of DNA containing complete Shiga-like toxin type I(sltI) operons from Escherichia coli strains belonging to serotypes O48:H21, O111:H- and OX3:Hs. These fragments were cloned and DNA sequence analysis identified several variations, as compared with published sltI sequences. All three sltI genes analysed were more closely related to Shiga toxin-encoding genes (sht) of Shigella dysenteriae type 1, than to previously published E. coli phage-encoded sltI genes. The greatest deviation in deduced amino acid (aa) sequence was observed in the SltI protein from the OX3:H8 strain, which differed from the phage 933J-encoded SltI by 9 aa in the A subunit and 3 aa in the B subunit.

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页码：71 / 74

页数：4

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共 15 条

[1] ALTERATION OF THE GLYCOLIPID BINDING-SPECIFICITY OF THE PIG EDEMA TOXIN FROM GLOBOTETRAOSYL TO GLOBOTRIASOYL CERAMIDE ALTERS INVIVO TISSUE TARGETING AND RESULTS IN A VEROTOXIN-1 LIKE DISEASE IN PIGS [J].

BOYD, B ;

TYRRELL, G ;

MALONEY, M ;

GYLES, C ;

BRUNTON, J ;

LINGWOOD, C .

JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (06) :1745-1753

[2] NUCLEOTIDE-SEQUENCE OF THE SHIGA-LIKE TOXIN GENES OF ESCHERICHIA-COLI [J].

CALDERWOOD, SB ;

AUCLAIR, F ;

DONOHUEROLFE, A ;

KEUSCH, GT ;

MEKALANOS, JJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (13) :4364-4368

[3] NUCLEOTIDE-SEQUENCE AND PROMOTER MAPPING OF THE ESCHERICHIA-COLI SHIGA-LIKE TOXIN OPERON OF BACTERIOPHAGE-H-19B [J].

DEGRANDIS, S ;

GINSBERG, J ;

TOONE, M ;

CLIMIE, S ;

FRIESEN, J ;

BRUNTON, J .

JOURNAL OF BACTERIOLOGY, 1987, 169 (09) :4313-4319

[4] MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF ANOTHER VARIANT OF THE ESCHERICHIA-COLI SHIGA-LIKE TOXIN-II FAMILY [J].

GANNON, VPJ ;

TEERLING, C ;

MASRI, SA ;

GYLES, CL .

JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :1125-1135

[5] CLONING AND NUCLEOTIDE-SEQUENCE ANALYSIS OF THE GENES DETERMINING VEROCYTOTOXIN PRODUCTION IN A PORCINE EDEMA DISEASE ISOLATE OF ESCHERICHIA-COLI [J].

GYLES, CL ;

DEGRANDIS, SA ;

MACKENZIE, C ;

BRUNTON, JL .

MICROBIAL PATHOGENESIS, 1988, 5 (06) :419-426

[6] CLONING AND NUCLEOTIDE SEQUENCING OF VERO TOXIN 2 VARIANT GENES FROM ESCHERICHIA-COLI O91-H21 ISOLATED FROM A PATIENT WITH THE HEMOLYTIC UREMIC SYNDROME [J].

ITO, H ;

TERAI, A ;

KURAZONO, H ;

TAKEDA, Y ;

NISHIBUCHI, M .

MICROBIAL PATHOGENESIS, 1990, 8 (01) :47-60

[7] NUCLEOTIDE-SEQUENCE ANALYSIS AND COMPARISON OF THE STRUCTURAL GENES FOR SHIGA-LIKE TOXIN-I AND SHIGA-LIKE TOXIN-II ENCODED BY BACTERIOPHAGES FROM ESCHERICHIA-COLI 933 [J].

JACKSON, MP ;

NEILL, RJ ;

OBRIEN, AD ;

HOLMES, RK ;

NEWLAND, JW .

FEMS MICROBIOLOGY LETTERS, 1987, 44 (01) :109-114

[8] THE PRIMARY STRUCTURE OF THE OPERONS CODING FOR SHIGELLA-DYSENTERIAE TOXIN AND TEMPERATE PHAGE H30 SHIGA-LIKE TOXIN [J].

KOZLOV, YV ;

KABISHEV, AA ;

LUKYANOV, EV ;

BAYEV, AA .

GENE, 1988, 67 (02) :213-221

[9] SHIGA-LIKE TOXIN-CONVERTING PHAGES FROM ESCHERICHIA-COLI STRAINS THAT CAUSE HEMORRHAGIC COLITIS OR INFANTILE DIARRHEA [J].

OBRIEN, AD ;

NEWLAND, JW ;

MILLER, SF ;

HOLMES, RK ;

SMITH, HW ;

FORMAL, SB .

SCIENCE, 1984, 226 (4675) :694-696

[10] SHIGA AND SHIGA-LIKE TOXINS [J].

OBRIEN, AD ;

HOLMES, RK .

MICROBIOLOGICAL REVIEWS, 1987, 51 (02) :206-220