DISTRIBUTION OF PLASMA-MEMBRANE CA2+-ATPASE AND INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR IN HUMAN PLATELET MEMBRANES

被引:24
作者
DEAN, WL
QUINTON, TM
机构
[1] Department of Biochemistry, University of Louisville School of Medicine, Louisville, KY
关键词
D O I
10.1016/0143-4160(95)90103-5
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Human platelet plasma membranes were prepared by the glycerol lysis method of Harmon et al. [Harmon JT. Greco NJ. Jamieson GA. (1992) Isolation of human platelet plasma membranes by glycerol lysis. Meth, Enzymol., 215, 32-36]. The membranes were observed to contain a Ca2+-ATPase with different properties than those of internal membranes. The specific activity of Ca2+-ATPase was lower in plasma membranes (10-40 nmol ATP hydrolyzed/min/mg), but the ATPase was less sensitive to thapsigargin (41% inhibition at 500 nM) and more sensitive to vanadate (50% inhibition at 4 mu M) than the Ca2+-ATPase in internal platelet membranes. The plasma membranes contained a Ca2+-ATPase detectable by monoclonal and polyclonal antibodies against erythrocyte Ca2+-ATPase that had a molecular mass of 144 kD, However, an anti-peptide antibody against an N-terminal sequence of the inositol 1,4,5-trisphosphate receptor recognized this protein in internal membranes, but not plasma membranes.
引用
收藏
页码:65 / 70
页数:6
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