INTRACELLULAR CALCIUM RELEASE IN N1E-115 NEUROBLASTOMA-CELLS IS MEDIATED BY THE M1 MUSCARINIC RECEPTOR SUBTYPE AND IS ANTAGONIZED BY MCN-A-343

被引：13

作者：

MATHES, C

WANG, SSH

VARGAS, HM

THOMPSON, SH

机构：

[1] UNIV CALIF LOS ANGELES,BRAIN RES INST,DEPT PHARMACOL,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,BRAIN RES INST,INTERDEPT PROGRAM NEUROSCI,LOS ANGELES,CA 90024

来源：

BRAIN RESEARCH | 1992年 / 585卷 / 1-2期

关键词：

MUSCARINIC RECEPTOR SUBTYPE; 4-DAMP; AFDX116; PIRENZEPINE; MCN-A-343; (+/-)BN228(4-[N]-(CHLOROPHENYL)CARBOXYLOXY-PENT-2-YNYLTRIMETHYLAMMONIUM BR); CALCIUM RELEASE; N1E-115; NEUROBLASTOMA;

D O I：

10.1016/0006-8993(92)91225-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtype(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (+/-)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP > pirenzepine > AFDX116 > (+/-)BN228 and McN-A-343. This profile, pirenzepine's high potency (19-fold greater than AFDX116) and its IC50 of 31 nM suggest that calcium release in this neuronal cell line is mediated by the M1 muscarinic receptor subtype.

引用

页码：307 / 310

页数：4

共 19 条

[11] CLONING, SEQUENCING AND EXPRESSION OF COMPLEMENTARY-DNA ENCODING THE MUSCARINIC ACETYLCHOLINE-RECEPTOR [J].