CHARACTERIZATION OF A PUTATIVE CELLULAR RECEPTOR FOR HIV-1 TRANSMEMBRANE GLYCOPROTEIN USING SYNTHETIC PEPTIDES

被引：74

作者：

QURESHI, NM

COY, DH

GARRY, RF

HENDERSON, LA

机构：

[1] TULANE UNIV, SCH MED, DEPT PATHOL, 1430 TULANE AVE, NEW ORLEANS, LA 70112 USA

[2] TULANE UNIV, SCH MED, DEPT MICROBIOL & IMMUNOL, NEW ORLEANS, LA 70112 USA

[3] TULANE UNIV, SCH MED, DEPT MED, NEW ORLEANS, LA 70112 USA

来源：

AIDS | 1990年 / 4卷 / 06期

关键词：

Cytopathology; HIV-1; Receptor; Therapy; Transmembrane glycoprotein;

D O I：

10.1097/00002030-199006000-00009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The transmembrane glycoprotein (gp41 or TM) of HIV-1 contains limited sequence similarity to TM of some immunosuppressive animal retroviruses. A specific HIV-1 TM sequence, denoted CS3, inhibits T-cell activation in vitro and antibody specific to CS3 has been linked to the absence of disease. CS3, when conjugated to human serum albumin (HSA) and labeled with fluorescein, binds specifically to CD4+ cell lines. Cross-linking of CS3-HSA to its binding activity on the CD4+ cell line RH9 reveals a putative subunit size of approximately 44 kD. Incubation of RH9 cells with CS3-HSA prior to addition of HIV-1 prevented HIV-1-mediated cell lysis and inhibited infection. These results suggest that the CS3 region of TM plays an important role in the pathogenesis of the AIDS virus, HIV-1.

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收藏

页码：553 / 558

页数：6

相关论文

共 22 条

[11] SELECTIVE TROPISM OF LYMPHADENOPATHY ASSOCIATED VIRUS (LAV) FOR HELPER-INDUCER LYMPHOCYTES-T [J].

KLATZMANN, D ;

BARRESINOUSSI, F ;

NUGEYRE, MT ;

DAUGUET, C ;

VILMER, E ;

GRISCELLI, C ;

BRUNVEZINET, F ;

ROUZIOUX, C ;

GLUCKMAN, JC ;

CHERMANN, JC ;

MONTAGNIER, L .

SCIENCE, 1984, 225 (4657) :59-63

[12] ANTIBODIES TO AN HIV-1 SYNTHETIC PEPTIDE WITH PARTIAL HOMOLOGY TO MULV P15E DO NOT PROTECT AGAINST AIDS [J].

LANGE, J ;

VAHLNE, A ;

DEKKER, J ;

DEWOLF, F ;

GOUDSMIT, J .

AIDS, 1989, 3 (06) :402-403

[13] AIDS RETROVIRUS (ARV-2) CLONE REPLICATES IN TRANSFECTED HUMAN AND ANIMAL FIBROBLASTS [J].

LEVY, JA ;

CHENGMAYER, C ;

DINA, D ;

LUCIW, PA .

SCIENCE, 1986, 232 (4753) :998-1001

[14] INDUCTION OF CD4-DEPENDENT CELL-FUSION BY THE HTLV-III/LAV ENVELOPE GLYCOPROTEIN [J].

LIFSON, JD ;

FEINBERG, MB ;

REYES, GR ;

RABIN, L ;

BANAPOUR, B ;

CHAKRABARTI, S ;

MOSS, B ;

WONGSTAAL, F ;

STEIMER, KS ;

ENGLEMAN, EG .

NATURE, 1986, 323 (6090) :725-728

[15] AIDS RETROVIRUS INDUCED CYTOPATHOLOGY - GIANT-CELL FORMATION AND INVOLVEMENT OF CD4 ANTIGEN [J].

LIFSON, JD ;

REYES, GR ;

MCGRATH, MS ;

STEIN, BS ;

ENGLEMAN, EG .

SCIENCE, 1986, 232 (4754) :1123-1127

[16] THE T4 GENE ENCODES THE AIDS VIRUS RECEPTOR AND IS EXPRESSED IN THE IMMUNE-SYSTEM AND THE BRAIN [J].

MADDON, PJ ;

DALGLEISH, AG ;

MCDOUGAL, JS ;

CLAPHAM, PR ;

WEISS, RA ;

AXEL, R .

CELL, 1986, 47 (03) :333-348

[17] BINDING OF HTLV-III/LAV TO T4+ T-CELLS BY A COMPLEX OF THE 110K VIRAL PROTEIN AND THE T4 MOLECULE [J].

MCDOUGAL, JS ;

KENNEDY, MS ;

SLIGH, JM ;

CORT, SP ;

MAWLE, A ;

NICHOLSON, JKA .

SCIENCE, 1986, 231 (4736) :382-385

[18] COMPLETE NUCLEOTIDE-SEQUENCE OF THE AIDS VIRUS, HTLV-III [J].

RATNER, L ;

HASELTINE, W ;

PATARCA, R ;

LIVAK, KJ ;

STARCICH, B ;

JOSEPHS, SF ;

DORAN, ER ;

RAFALSKI, JA ;

WHITEHORN, EA ;

BAUMEISTER, K ;

IVANOFF, L ;

PETTEWAY, SR ;

PEARSON, ML ;

LAUTENBERGER, JA ;

PAPAS, TS ;

GHRAYEB, J ;

CHANG, NT ;

GALLO, RC ;

WONGSTAAL, F .

NATURE, 1985, 313 (6000) :277-284

[19] THE RESERVOIR FOR HIV-1 IN HUMAN PERIPHERAL-BLOOD IS A T-CELL THAT MAINTAINS EXPRESSION OF CD4 [J].

SCHNITTMAN, SM ;

PSALLIDOPOULOS, MC ;

LANE, HC ;

THOMPSON, L ;

BASELER, M ;

MASSARI, F ;

FOX, CH ;

SALZMAN, NP ;

FAUCI, AS .

SCIENCE, 1989, 245 (4915) :305-308

[20] IMMUNOSUPPRESSIVE ACTIVITY OF THE RETROVIRAL ENVELOPE PROTEIN-P15E AND ITS POSSIBLE RELATIONSHIP TO NEOPLASIA [J].

SNYDERMAN, R ;

CIANCIOLO, GJ .

IMMUNOLOGY TODAY, 1984, 5 (08) :240-244