RECOVERY OF MITOGENIC ACTIVITY OF A GROWTH-FACTOR MUTANT WITH A NUCLEAR TRANSLOCATION SEQUENCE

被引：365

作者：

IMAMURA, T ^{[1
]}

ENGLEKA, K ^{[1
]}

ZHAN, X ^{[1
]}

TOKITA, Y ^{[1
]}

FOROUGH, R ^{[1
]}

ROEDER, D ^{[1
]}

JACKSON, A ^{[1
]}

MAIER, JAM ^{[1
]}

HLA, T ^{[1
]}

MACIAG, T ^{[1
]}

机构：

[1] AMER RED CROSS,JEROME H HOLLAND LAB BIOMED SCI,MOLEC BIOL LAB,15601 CRABBS BRANCH WAY,ROCKVILLE,MD 20855

来源：

SCIENCE | 1990年 / 249卷 / 4976期

关键词：

D O I：

10.1126/science.1699274

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Heparin-binding growth factor-1 (HBGF-1) is an angiogenic polypeptide mitogen for mesoderm- and neuroectoderm-derived cells in vitro and remains biologically active after truncation of the amino-terminal domain (HBGF-1α) of the HBGF-1β precursor. Polymerase chain reaction mutagenesis and prokaryotic expression systems were used to prepare a mutant of HBGF-1α lacking a putative nuclear translocation sequence (amino acid residues 21 to 27; HBGF-1U). Although HBGF-1U retains its ability to bind to heparin, HBGF-1U fails to induce DNA synthesis and cell proliferation at concentrations sufficient to induce intracellular receptor-mediated tyrosine phosphorylation and c-fos expression. Attachment of the nuclear translocation sequence from yeast histone 2B at the amino terminus of HBGF-1U yields a chimeric polypeptide (HBGF-1U2) with mitogenic activity in vitro and indicates that nuclear translocation is important for this biological response.

引用

页码：1567 / 1570

页数：4

共 55 条

[11] BIOLOGY OF INTERLEUKIN-1 [J].