ISOLATION AND STRUCTURAL CHARACTERIZATION OF NOVEL SIALYLATED OLIGOSACCHARIDE-ALDITOLS FROM RESPIRATORY-MUCUS GLYCOPROTEINS OF A PATIENT SUFFERING FROM BRONCHIECTASIS

The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group 0) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169-1821. In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC. From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz H-1-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis.