SEPARATION AND DETERMINATION OF LIVER URIC-ACID AND ALLANTOIN

被引：6

作者：

TERZUOLI, L ^{[1
]}

PANDOLFI, M ^{[1
]}

AREZZINI, L ^{[1
]}

PIZZICHINI, M ^{[1
]}

MARINELLO, E ^{[1
]}

PAGANI, R ^{[1
]}

机构：

[1] UNIV SIENA,IST BIOCHIM & ENZIMOL,I-53100 SIENA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 663卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00420-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously described the only satisfactory procedure yet achieved for separating uric acid and allantoin from rat liver. The procedure was based on trichloroacetic acid (TCA) extraction, acid hydrolysis, treatment with Hg-acetate, and cation- and anion-exchange chromatography. After separation, allantoin was quantified by a colorimetric method, and uric acid enzymatically using uricase. Since this procedure is too time-consuming, we propose an improved version which avoids the need for anion-exchange chromatography and the complex assay of catabolic compounds. The new method consists of a very fast and simple HPLC separation and direct determination of uric acid and allantoin at 220 nm. The method can be used for fresh tissue or after treatment of the tissue with labeled precursor.

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页码：143 / 147

页数：5

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