MOBILIZATION OF RECOMBINANT PLASMIDS FROM STAPHYLOCOCCUS-AUREUS INTO COAGULASE NEGATIVE STAPHYLOCOCCUS SPECIES

被引：13

作者：

THOMAS, WD

ARCHER, GL

机构：

[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT MED,RICHMOND,VA 23298

[2] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT MICROBIOL IMMUNOL,RICHMOND,VA 23298

来源：

PLASMID | 1992年 / 27卷 / 02期

关键词：

D O I：

10.1016/0147-619X(92)90017-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

pC221, a small nonconjugative staphylococcal plasmid, can be mobilized between staphylococci by pG01, a larger conjugative plasmid. pC221 carries the two transacting genes, mobA and mobB, which are needed for its mobilization. The products of these genes create a site-specific single-stranded nick (mobA) and then facilitate DNA transfer (mobB). Several useful Escherichia coli-staphylococcal shuttle plasmids containing the cloned single-stranded nick site were created and successfully mobilized into Staphylococcus aureus and two coagulase-negative staphylococci, S. epidermidis and S. saprophyticus, by providing mob genes (pC221) and conjugative transfer genes (pG01) in trans in the donor. These vectors may offer a genetic system for the introduction of recombinant plasmids into coagulase negative staphylococci. © 1992.

引用

页码：164 / 168

页数：5

共 19 条

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