A METHOD FOR 2D CRYSTALLIZATION OF SOLUBLE-PROTEINS AT LIQUID-LIQUID INTERFACE

被引：27

作者：

AOYAMA, K

OGAWA, K

KIMURA, Y

FUJIYOSHI, Y

机构：

[1] MATSUSHITA ELECT IND CO LTD,INT INST ADV RES,KYOTO 61902,JAPAN

[2] JAPAN RES DEV CORP,ERATO,TONOMURA ELECTRON WAVEFRONT PROJECT,HATOYAMA,SAITAMA 35003,JAPAN

[3] IWATE UNIV,FAC AGR,DEPT VET ANAT,MORIOKA,IWATE 020,JAPAN

[4] PROT ENGN RES INST,SUITA,OSAKA 565,JAPAN

来源：

ULTRAMICROSCOPY | 1995年 / 57卷 / 04期

关键词：

D O I：

10.1016/0304-3991(94)00192-P

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Two-dimensional crystals of soluble proteins were formed at the interface between an aqueous solution of proteins and a thin organic liquid (dehydroabietylamine). Proteins were adsorbed to the interface from the aqueous side and formed a two-dimensional crystal under suitable conditions. This method offers the advantage of great surface mobility and ideal homogeneity. Furthermore, the positive charge attracted negatively charged proteins well to the interface and no denaturation of the proteins was observed. With this technique, two-dimensional crystals of ferritin, catalase, chaperonin and 50S ribosome were prepared and their structural features were determined.

引用

页码：345 / 354

页数：10

共 13 条

[11] HOLES IN A STEARIC-ACID MONOLAYER OBSERVED BY DARK-FIELD ELECTRON-MICROSCOPY
UYEDA, N
TAKENAKA, T
AOYAMA, K
MATSUMOTO, M
FUJIYOSHI, Y
[J]. NATURE, 1987, 327 (6120) : 319 - 321
[12] TWO-DIMENSIONAL CRYSTALLIZATION TECHNIQUE FOR IMAGING MACROMOLECULES, WITH APPLICATION TO ANTIGEN-ANTIBODY COMPLEMENT COMPLEXES
UZGIRIS, EE
KORNBERG, RD
[J]. NATURE, 1983, 301 (5896) : 125 - 129
[13] 2-DIMENSIONAL CRYSTALLIZATION OF PROTEINS ON MERCURY
YOSHIMURA, H
MATSUMOTO, M
ENDO, S
NAGAYAMA, K
[J]. ULTRAMICROSCOPY, 1990, 32 (03) : 265 - 274

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