CYP52 (CYTOCHROME-P450ALK) MULTIGENE FAMILY IN CANDIDA-MALTOSA - IDENTIFICATION AND CHARACTERIZATION OF 8 MEMBERS

被引：73

作者：

OHKUMA, M ^{[1
]}

MURAOKA, S ^{[1
]}

TANIMOTO, T ^{[1
]}

FUJII, M ^{[1
]}

OHTA, A ^{[1
]}

TAKAGI, M ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT AGR CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

DNA AND CELL BIOLOGY | 1995年 / 14卷 / 02期

关键词：

D O I：

10.1089/dna.1995.14.163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, we characterized three genes and presented evidence for an n-alkane-inducible cytochrome P450 (P450alk) multigene family in an n-alkane-assimilating and diploid-type yeast, Candida maltosa. In the present report, we isolated and characterized additional members of this gene family, including a total of thirteen P450alk-related sequences (eight genes and five of their alleles). Two sets, each consisting of two genes, were tandemly arranged in the genome. A gene replacement experiment showed that at least one gene had only a single allele in the genome. The determined nucleotide and the deduced amino acid sequences indicated that all had a characteristic constituent for P450s and exhibited amino acid identities from 94% to 37% to each other. Six genes showed relatively higher similarities to each other than to the other two genes and were thus classified into a subfamily. All the members of this subfamily were assigned to the same single chromosome, showing a good correlation between sequence similarity and chromosomal linkage. Although all the genes except for one were induced by n-alkane, their inducibilities by some other aliphatic carbon sources showed variabilities.

引用

页码：163 / 173

页数：11

共 32 条

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