DNA STRAND BREAKAGE AND BASE MODIFICATION INDUCED BY HYDROGEN-PEROXIDE TREATMENT OF HUMAN RESPIRATORY-TRACT EPITHELIAL-CELLS

被引：52

作者：

SPENCER, JPE

JENNER, A

CHIMEL, K

ARUOMA, OI

CROSS, CE

WU, R

HALLIWELL, B

机构：

[1] UNIV CALIF DAVIS,CALIF REG PRIMATE RES CTR,PULM RES GRP,DAVIS,CA 95616

[2] UNIV CALIF DAVIS,MED CTR,DEPT INTERNAL MED,DIV PULM CRIT CARE MED,SACRAMENTO,CA 95817

来源：

FEBS LETTERS | 1995年 / 374卷 / 02期

关键词：

HUMAN RESPIRATORY TRACT EPITHELIAL CELL; DNA DAMAGE; DNA BASE MODIFICATION; STRAND BREAKAGE; H2O2; GC-MS;

D O I：

10.1016/0014-5793(95)01117-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of human respiratory tract epithelial cells with H2O2 led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH., However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.

引用

页码：233 / 236

页数：4

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