MAPPING THE TRANSACTIVATION DOMAIN OF THE OCT-6 POU TRANSCRIPTION FACTOR

被引：46

作者：

MEIJER, D ^{[1
]}

GRAUS, A ^{[1
]}

GROSVELD, G ^{[1
]}

机构：

[1] ERASMUS UNIV, DEPT CELL BIOL & GENET, MGC, 3000 DR ROTTERDAM, NETHERLANDS

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 09期

关键词：

D O I：

10.1093/nar/20.9.2241

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The POU transcription factor Oct-6 is expressed in embryonic stem cells, glial progenitor cells and in a restricted set of neurons in the CNS. The protein has been shown to act as a transactivator as well as a repressor. Here we show that the Oct-6 protein activates transcription from three different promoters in HeLa cells. The ability to activate a minimal tk promoter via a multimerized IgH enhancer octamer motif relies on a domain within the aminoterminal third of the protein. Parts of this domain can be deleted without abolishing transactivation, suggesting that there is functional redundancy within this region. The transactivation domain of the Oct-6 protein is different from other described activation domains in that it is highly glycine and alanine rich.

引用

页码：2241 / 2247

页数：7

共 40 条

[1] THE B-CELL-SPECIFIC OCT-2 PROTEIN CONTAINS POU BOX-TYPE AND HOMEO BOX-TYPE DOMAINS [J].

CLERC, RG ;

CORCORAN, LM ;

LEBOWITZ, JH ;

BALTIMORE, D ;

SHARP, PA .

GENES & DEVELOPMENT, 1988, 2 (12A) :1570-1581

[2] ISOLATION OF COACTIVATORS ASSOCIATED WITH THE TATA-BINDING PROTEIN THAT MEDIATE TRANSCRIPTIONAL ACTIVATION [J].

DYNLACHT, BD ;

HOEY, T ;

TJIAN, R .

CELL, 1991, 66 (03) :563-576

[3] THE CELL TYPE-SPECIFIC OCTAMER TRANSCRIPTION FACTOR OTF-2 HAS 2 DOMAINS REQUIRED FOR THE ACTIVATION OF TRANSCRIPTION [J].

GERSTER, T ;

BALMACEDA, CG ;

ROEDER, RG .

EMBO JOURNAL, 1990, 9 (05) :1635-1643

[4]

Gorman C., 1985, DNA CLONING PRACTICA, V1, P143

[5] TST-1, A MEMBER OF THE POU DOMAIN GENE FAMILY, BINDS THE PROMOTER OF THE GENE ENCODING THE CELL-SURFACE ADHESION MOLECULE PO [J].

HE, X ;

GERRERO, R ;

SIMMONS, DM ;

PARK, RE ;

LIN, CJR ;

SWANSON, LW ;

ROSENFELD, MG .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (03) :1739-1744

[6] THE POU DOMAIN - A LARGE CONSERVED REGION IN THE MAMMALIAN PIT-1, OCT-1, OCT-2, AND CAENORHABDITIS-ELEGANS UNC-86 GENE-PRODUCTS [J].

HERR, W ;

STURM, RA ;

CLERC, RG ;

CORCORAN, LM ;

BALTIMORE, D ;

SHARP, PA ;

INGRAHAM, HA ;

ROSENFELD, MG ;

FINNEY, M ;

RUVKUN, G ;

HORVITZ, HR .

GENES & DEVELOPMENT, 1988, 2 (12A) :1513-1516

[7] STRINGENT INTEGRITY REQUIREMENTS FOR BOTH TRANSACTIVATION AND DNA-BINDING IN A TRANSACTIVATOR, OCT3 [J].

IMAGAWA, M ;

MIYAMOTO, A ;

SHIRAKAWA, M ;

HAMADA, H ;

MURAMATSU, M .

NUCLEIC ACIDS RESEARCH, 1991, 19 (16) :4503-4508

[8] THE POU-SPECIFIC DOMAIN OF PIT-1 IS ESSENTIAL FOR SEQUENCE-SPECIFIC, HIGH-AFFINITY DNA-BINDING AND DNA-DEPENDENT PIT-1-PIT-1 INTERACTIONS [J].

INGRAHAM, HA ;

FLYNN, SE ;

VOSS, JW ;

ALBERT, VR ;

KAPILOFF, MS ;

WILSON, L ;

ROSENFELD, MG .

CELL, 1990, 61 (06) :1021-1033

[9] OCTAMER TRANSCRIPTION FACTORS AND THE CELL TYPE-SPECIFICITY OF IMMUNOGLOBULIN GENE-EXPRESSION [J].

KEMLER, I ;

SCHAFFNER, W .

FASEB JOURNAL, 1990, 4 (05) :1444-1449

[10]

LATHANGUE NB, 1988, TRANSCRIPTION SPLICI, P1

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