PRIMARY STRUCTURE OF THE HUMAN MUSCLE ACETYLCHOLINE-RECEPTOR - CDNA CLONING OF THE GAMMA-SUBUNIT AND EPSILON-SUBUNIT

被引：81

作者：

BEESON, D ^{[1
]}

BRYDSON, M ^{[1
]}

BETTY, M ^{[1
]}

JEREMIAH, S ^{[1
]}

POVEY, S ^{[1
]}

VINCENT, A ^{[1
]}

NEWSOMDAVIS, J ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,GALTON LAB,MRC,HUMAN BIOCHEM GENET UNIT,LONDON WC1E 6BT,ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 215卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb18027.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA sequences encompassing the full coding region for the human muscle acetylcholine receptor (AChR) epsilon and gamma subunits have been isolated. The deduced amino-acid sequences indicate that the mature epsilon subunit contains 473 amino acids and is preceded by a 20-amino-acid signal peptide. As predicted from genomic clones, the gamma subunit contains 495 amino acids preceded by a 22-amino-acid signal peptide. In common with the human alpha, beta, gamma and delta subunits the epsilon subunit is highly conserved between mammalian species. The epsilon subunit gene is not closely linked to the gamma and delta subunits on chromosome 2 but rather is located with the beta subunit on chromosome 17. Expression of the alpha-, beta-, gamma-, delta- and epsilon-subunit cRNAs in rabbit-reticulocyte lysates followed by analysis on SDS/PAGE show glycosylated proteins with apparent molecular masses of 44-60 kDa.

引用

页码：229 / 238

页数：10

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