SEQUENCE AND CHARACTERIZATION OF THE ESCHERICHIA-COLI GENOME BETWEEN THE NDK AND GCPE GENES

被引：2

作者：

BAKER, J ^{[1
]}

PARKER, J ^{[1
]}

机构：

[1] SO ILLINOIS UNIV,DEPT MICROBIOL,CARBONDALE,IL 62901

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 121卷 / 03期

关键词：

ESCHERICHIA COLI; OPEN READING FRAME; GENOMIC SEQUENCE;

D O I：

10.1111/j.1574-6968.1994.tb07115.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The region of the chromosome immediately upstream of the Escherichia coli gene gcpE has been cloned and sequenced. This region contains two functional open reading frames, orf 384 and orf 337, encoding proteins of 43082 and 36189 Da, respectively. Sequencing analysis (this paper) and the isolation of a DNA fragment containing a functional promoter (Talukder, A.A., Yanai, S., and Yamada, M. (1994) Biosci. Biotech. Biochem. 58, 117-120) indicate that orf 337 is in an operon with gcpE. The gene orf 384 is immediately downstream of the gene ndk, which encodes nucleoside diphosphate kinase.

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页码：293 / 296

页数：4

相关论文

共 15 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2]

BAKER J, 1992, FEMS MICROBIOL LETT, V94, P175

[3]

BANKER GA, 1972, J BIOL CHEM, V247, P5856

[4]

FREEDMAN R, 1985, J BIOL CHEM, V260, P10063

[5] NUCLEOSIDE DIPHOSPHATE KINASE FROM ESCHERICHIA-COLI - ITS OVERPRODUCTION AND SEQUENCE COMPARISON WITH EUKARYOTIC ENZYMES [J].

HAMA, H ;

ALMAULA, N ;

LERNER, CG ;

INOUYE, S ;

INOUYE, M .

GENE, 1991, 105 (01) :31-36

[6]

HENIKOFF S, 1987, METHOD ENZYMOL, V155, P156

[7] CORRELATION BETWEEN THE ABUNDANCE OF ESCHERICHIA-COLI TRANSFER-RNAS AND THE OCCURRENCE OF THE RESPECTIVE CODONS IN ITS PROTEIN GENES - A PROPOSAL FOR A SYNONYMOUS CODON CHOICE THAT IS OPTIMAL FOR THE ESCHERICHIA-COLI TRANSLATIONAL SYSTEM [J].

IKEMURA, T .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 151 (03) :389-409

[8] THE PHYSICAL MAP OF THE WHOLE ESCHERICHIA-COLI CHROMOSOME - APPLICATION OF A NEW STRATEGY FOR RAPID ANALYSIS AND SORTING OF A LARGE GENOMIC LIBRARY [J].

KOHARA, Y ;

AKIYAMA, K ;

ISONO, K .

CELL, 1987, 50 (03) :495-508

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] SINGLE-STRANDED-DNA BLUE-T7 PROMOTER PLASMIDS - A VERSATILE TANDEM PROMOTER SYSTEM FOR CLONING AND PROTEIN ENGINEERING [J].

MEAD, DA ;

SZCZESNASKORUPA, E ;

KEMPER, B .

PROTEIN ENGINEERING, 1986, 1 (01) :67-74