STRUCTURE AND INTERACTION OF INHIBITORS WITH THE TEA H+ EXCHANGER OF RABBIT RENAL BRUSH-BORDER MEMBRANES

被引:32
作者
WRIGHT, SH
WUNZ, TM
WUNZ, TP
机构
[1] Department of Physiology, College of Medicine, University of Arizona, Tucson, 85724, AZ
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1995年 / 429卷 / 03期
关键词
TRANSPORT; KIDNEY; ORGANIC CATION;
D O I
10.1007/BF00374145
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The renal secretion of organic cations (OCs) involves a carrier-mediated exchange of OC for H+ in the luminal membrane of proximal cells. To assess the influence of chemical structure on the interaction of potential substrates with this process we examined the effect of a series of quaternary ammonium compounds on the transport of the OC tetraethylammonium (TEA) in a preparation of isolated renal brush-border membrane vesicles. Apparent inhibitory potency varied over a factor of 10(4), as expressed in inhibitor coefficients (K-i(TEA)) whose approximate values ranged from 0.5 mu M to 5 mM. The poorest inhibitors of TEA/H+ exchange were those molecules with carboxyl or hydroxyl residues, whereas the addition of methylene groups to a parent molecule tended to increase inhibitory potency. A plot of apparent K-i(TEA) versus calculated octanol:water partition coefficient (expressed in terms of a relative lipophilicity factor) showed a clear correlation between these two parameters, although there was considerable variability between apparent lipophilicity and K-i(TEA) for molecules with very different parent structures. For select groups of molecules with similar parent structures (e.g., the n-tetraalkylammoniums or the 4-phenylpyridinium, 3-phenylpyridinium, and quinolinium compounds) the correlation between calculated lipophilicity and apparent K-i(TEA) was more marked. However, even within these groups of closely related parent structures, there appeared to be subtle, but systematic, variations in inhibitory potency that may have been related to the influence of steric factors on the binding of inhibitors to the TEA/H+ exchanger. We conclude that the lipophilic nature of a quaternary ammonium compound represents the predominant factor in the binding to, and subsequent inhibition of, luminal TEA/H+ exchange. Specific steric factors may influence the binding of substrate to the exchanger, but play a secondary role in this interaction.
引用
收藏
页码:313 / 324
页数:12
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