DETERMINATION OF VITAMIN-B12 IN MULTIVITAMIN MULTIMINERAL TABLETS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER SOLID-PHASE EXTRACTION

被引：25

作者：

DALBACKE, J

DAHLQUIST, I

机构：

[1] Analytical and Quality Development Department, Gacell Laboratories AB, S-201 80 Malmö

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 541卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)96010-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of vitamin B12 (cyanocobalamin) in multivitamin-multimineral tablets, containing 3-mu-g of vitamin B12 per tablet, is described. Powdered tablets are extracted with a mixture of ammonium pyrrolidinedithiocarbamate and citric acid in dimethyl sulphoxide and water. The extract is centrifuged and the supernatant is diluted with water before concentration and clean-up by solid-phase extraction using a quaternary amine and a phenyl column in series. The eluate from the solid-phase extraction is analysed by reversed-phase liquid chromatography using a methanol-water gradient with detection at 550 nm. A comparison with the frequently used microbiological method is presented. The validation of the procedure is described in terms of selectivity, linearity, accuracy and precision.

引用

页码：383 / 392

页数：10