CLONING AND SEQUENCE-ANALYSIS OF THE COAT PROTEIN GENE OF BARLEY MILD MOSAIC-VIRUS

被引：16

作者：

FOULDS, IJ

LEA, VJ

SIDEBOTTOM, C

JAMES, CM

BOULTON, RE

BREARS, T

SLABAS, AR

JACK, PL

STRATFORD, R

机构：

[1] PLANT BREEDING INT,MARIS LANE,CAMBRIDGE CB2 2LQ,ENGLAND

[2] UNILEVER RES LABS,SHARNBROOK MK44 1LQ,BEDS,ENGLAND

[3] ROCKEFELLER UNIV,PLANT MOLEC BIOL LAB,NEW YORK,NY 10021

来源：

VIRUS RESEARCH | 1993年 / 27卷 / 01期

关键词：

BAMMV; BAYMV; BYMOVIRUS; POTYVIRUS;

D O I：

10.1016/0168-1702(93)90114-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sequence of the 3' 1462nts of RNA-1 of a UK isolate of the fungal-transmitted virus barley mild mosaic (BaMMV) has been determined. An open reading frame encoding the coat protein gene was identified within this region using amino acid sequence information obtained by cyanogen bromide cleavage of virus particles. The amino acid sequence of the full-length coat protein was deduced from the nucleotide sequence. Amino acid sequence comparisons revealed highest homology to the coat protein of barley yellow mosaic virus. In addition, a significant, but limited, number of the amino acid residues that are conserved between aphid-transmitted potyviruses were also conserved between BaMMV and potyviruses.

引用

页码：79 / 89

页数：11

共 31 条

[11] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS [J].