INCIDENCE AND SPECIFICITY OF HLA-DP ANTIBODIES IN PREGNANCY SERA

被引：11

作者：

MUELLERECKHARDT, G

KIEFEL, V

TLUSTY, A

SCHOLTEN, J

SCHMIDT, A

MUELLERECKHARDT, C

机构：

[1] Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University Giessen

来源：

HUMAN IMMUNOLOGY | 1990年 / 29卷 / 03期

关键词：

D O I：

10.1016/0198-8859(90)90112-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A total of 630 human pregnancy sera were investigated for HLA-DP antibodies (ab) by monoclonal antibody-specific immobilization of leukocyte antigens (MAILA) using monoclonal antibody (mAB) B7/21.2 and selected B-lymphoblastoid cell lines from the Tenth International Histocompatibility Workshop reference panel. DP-specific abs were detected in 86 of 330 sera (26.1%) of a retrospective series selected for positive reactions in lymphocytotoxicity screening, and in 29 of 300 unselected sera (9.7%) of a prospective series. Approximately 80% of DP-reactive sera were also positive in lymphocytotoxicity test for class I and/or class II ab. On the other hand, sera containing lymphocytotoxic ab for both class I and class II ab revealed the highest incidence of DP ab (35%). Out of 115 DP-reactive sera, 28 clearly presented one or more DP specificities. Absorption/elution studies revealed complex patterns of reactivity in some sera which were similar to those of known mABs and possibly reflect supertypic DP specificities and/or serological cross-reactions. Serological DP typing of individuals by the MAILA technique appears promising. © 1990.

引用

页码：166 / 174

页数：9

共 33 条

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JOHNSON AH, 1989, IMMUNOBIOLOGY HLA, V1, P286

[12]

KIEFEL V, 1987, BLOOD, V70, P1722

[13] A SUPERTYPIC HLA-DP SPECIFICITY DEFINED BY 2 HUMAN-HUMAN HYBRIDOMA ANTIBODIES (TRB50-TRE11) [J].