DIRECT ASSOCIATION OF MESSENGER RNA-CONTAINING RIBONUCLEOPROTEIN-PARTICLES WITH MEMBRANES OF THE ENDOPLASMIC-RETICULUM IN ETHIONINE-TREATED RAT-LIVER

被引:9
作者
ENDO, Y
NATORI, Y
机构
[1] Department of Nutritional Chemistry, Tokushima University School of Medicine, Tokushima
关键词
(Endoplasmic reticulum; Rat liver); Ethionine; Membrane-bound mRNA; Polysome breakdown; Ribonucleoprotein;
D O I
10.1016/0005-2787(79)90173-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The administration of ethionine to female rats causes breakdown of hepatic polysomes. The fate of the mRNA molecules after polysome breakdown was investigated by measuring the amount of poly(A)-containing mRNA in membranous and non-membranous fractions obtained from the cytoplasm of ethionine-treated rat liver. The amount of poly(A)-containing mRNA in the membrane fraction of ethionine-treated liver was found to be the same as that of normal liver. When poly(A)-containing mRNAs from various fractions were translated in a wheat germ system and the products were isolated by immunoprecipitation, the albumin-specific mRNA was found exclusively in the membrane fraction of both normal and ethionine-treated livers. The membrane-bound mRNA in ethionine-treated liver, selectively labeled with [14C]orotate, was banded in CsCl gradient centrifugation at 1.42 g/ml which corresponds to the previously reported mRNA-containing ribonucleoprotein particles. From these results, we concluded that even after the polysome disaggregation by ethionine, most of the mRNA of membrane-bound polysomes remains attached to the endoplasmic reticulum membranes independently of ribosomes and the nascent polypeptide chains. © 1979.
引用
收藏
页码:281 / 291
页数:11
相关论文
共 28 条
[1]   RIBOSOME-MEMBRANE INTERACTION - NONDESTRUCTIVE DISASSEMBLY OF RAT-LIVER ROUGH MICROSOMES INTO RIBOSOMAL AND MEMBRANOUS COMPONENTS [J].
ADELMAN, MR ;
SABATINI, DD ;
BLOBEL, G .
JOURNAL OF CELL BIOLOGY, 1973, 56 (01) :206-229
[3]   CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONS .1. LOCATION OF POLYPEPTIDES WITHIN RIBOSOMES [J].
BLOBEL, G ;
SABATINI, DD .
JOURNAL OF CELL BIOLOGY, 1970, 45 (01) :130-&
[4]   TRANSFER OF PROTEINS ACROSS MEMBRANES .2. RECONSTITUTION OF FUNCTIONAL ROUGH MICROSOMES FROM HETEROLOGOUS COMPONENTS [J].
BLOBEL, G ;
DOBBERSTEIN, B .
JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :852-862
[5]   TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].
BLOBEL, G ;
DOBBERSTEIN, B .
JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851
[6]   RAPID EQUILIBRIUM ISOPYCNIC CSCL GRADIENTS [J].
BRUNK, CF ;
LEICK, V .
BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 179 (01) :136-&
[7]   DIRECT ASSOCIATION OF MESSENGER-RNA LABELED IN PRESENCE OF FLUORO-OROTATE WITH MEMBRANES OF ENDOPLASMIC-RETICULUM IN RAT-LIVER [J].
CARDELLI, J ;
LONG, B ;
PITOT, HC .
JOURNAL OF CELL BIOLOGY, 1976, 70 (01) :47-58
[8]   HOMOLOGY IN AMINO-TERMINAL SEQUENCE OF PRECURSORS TO PANCREATIC SECRETORY PROTEINS [J].
DEVILLERSTHIERY, A ;
KINDT, T ;
SCHEELE, G ;
BLOBEL, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (12) :5016-5020
[9]   STATE OF MESSENGER RIBONUCLEIC-ACID AND RIBOSOMES IN CYTOPLASM OF ETHIONINE-TREATED RAT-LIVER [J].
ENDO, Y ;
TOMINAGA, H ;
NATORI, Y .
BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 383 (03) :305-315
[10]   PRE-PROPARATHYROID HORMONE - ANALYSIS OF RADIOACTIVE TRYPTIC PEPTIDES AND AMINO-ACID SEQUENCE [J].
KEMPER, B ;
HABENER, JF ;
ERNST, MD ;
POTTS, JT ;
RICH, A .
BIOCHEMISTRY, 1976, 15 (01) :15-19