DIRECT ASSOCIATION OF MESSENGER RNA-CONTAINING RIBONUCLEOPROTEIN-PARTICLES WITH MEMBRANES OF THE ENDOPLASMIC-RETICULUM IN ETHIONINE-TREATED RAT-LIVER

被引:9
作者
ENDO, Y
NATORI, Y
机构
[1] Department of Nutritional Chemistry, Tokushima University School of Medicine, Tokushima
关键词
(Endoplasmic reticulum; Rat liver); Ethionine; Membrane-bound mRNA; Polysome breakdown; Ribonucleoprotein;
D O I
10.1016/0005-2787(79)90173-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The administration of ethionine to female rats causes breakdown of hepatic polysomes. The fate of the mRNA molecules after polysome breakdown was investigated by measuring the amount of poly(A)-containing mRNA in membranous and non-membranous fractions obtained from the cytoplasm of ethionine-treated rat liver. The amount of poly(A)-containing mRNA in the membrane fraction of ethionine-treated liver was found to be the same as that of normal liver. When poly(A)-containing mRNAs from various fractions were translated in a wheat germ system and the products were isolated by immunoprecipitation, the albumin-specific mRNA was found exclusively in the membrane fraction of both normal and ethionine-treated livers. The membrane-bound mRNA in ethionine-treated liver, selectively labeled with [14C]orotate, was banded in CsCl gradient centrifugation at 1.42 g/ml which corresponds to the previously reported mRNA-containing ribonucleoprotein particles. From these results, we concluded that even after the polysome disaggregation by ethionine, most of the mRNA of membrane-bound polysomes remains attached to the endoplasmic reticulum membranes independently of ribosomes and the nascent polypeptide chains. © 1979.
引用
收藏
页码:281 / 291
页数:11
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