QUANTITATIVE AND QUALITATIVE CHANGES IN RNA-METABOLISM IN RATS FED N-2-ACETYLAMINOFLUORENE

被引:4
作者
AUSTIN, GE
MOYER, GH
机构
[1] Department of Pathology, UCLA Center for the Health Sciences, Los Angeles
关键词
D O I
10.1016/0014-4800(79)90034-0
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The effects of N-2 acetylaminofluorene (AAF) on hepatic RNA metabolism were studied in rats fed the carcinogen for 3 to 31 days, a period when early events in neoplastic transformation are thought to occur. Although the liver cells remained normal microscopically, the rate of RNA synthesis was inhibited by 55% during the first week of AAF feeding. This resulted in a substantial decrease in both nuclear and cytoplasmic RNA. Shifts also occurred in the distribution of cytoplasmic RNA among various subcellular fractions. Sedimentation profiles showed a small but consistent reduction in polysome size and in the amount of RNA in the larger polysomal aggregates. All of these parameters returned to normal or near normal after 1 month of AAF feeding, indicating the development of a form of resistance to AAF. RNA-DNA hybridization experiments revealed that hepatic transcription of moderately reiterated genes throughout the first month of AAF feeding was qualitatively similar to that in normal liver. Likewise, transport of these classes of RNA to the cytoplasm was unaltered. The initial quantitative derangements in RNA metabolism and their subsequent recovery in the face of continued administration of the chemical may be important in the induction of hepatic cancer by AAF. © 1979.
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页码:333 / 343
页数:11
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