MORPHOLOGICAL AND FUNCTIONAL-CHARACTERIZATION OF ISOLATED HUMAN-PAROTID ACINAR-CELLS

被引：9

作者：

MANGOS, JA ^{[1
]}

机构：

[1] UNIV FLORIDA,J HILLIS MILLER HLTH CTR,DEPT BASIC DENT SCI,GAINESVILLE,FL 32610

来源：

JOURNAL OF DENTAL RESEARCH | 1979年 / 58卷 / 10期

关键词：

D O I：

10.1177/00220345790580101601

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

A method for the isolation of human parotid acinar cells using enzymatic dispersion and mild mechanical forces has been developed. The isolated acinar cells are morphologically and functionally intact, thus suitable for studies of the cellular physiology of secretion. © 1979, SAGE Publications. All rights reserved.

引用

页码：2028 / 2035

页数：8

共 8 条

[1] AMYLASES, ALPHA AND BETA [J].

BERNFELD, P .

METHODS IN ENZYMOLOGY, 1955, 1 :149-158

[2] CHOLINERGIC REGULATION OF CYCLIC NUCLEOTIDE LEVELS, AMYLASE RELEASE, AND K+ EFFLUX FROM RAT PAROTID-GLANDS [J].

BUTCHER, FR ;

MCBRIDE, PA ;

RUDICH, L .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1976, 5 (3-4) :243-254

[3] EFFECT OF ISOPROTERENOL AND ITS ANALOGS UPON ADENOSINE-3',5'-MONOPHOSPHATE AND GUANOSINE 3',5'-MONOPHOSPHATE LEVELS IN MOUSE PAROTID-GLAND INVIVO - RELATIONSHIP TO STIMULATION OF DNA-SYNTHESIS [J].

DURHAM, JP ;

BASERGA, R ;

BUTCHER, FR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 372 (01) :196-217

[4] DISPERSED RAT PAROTID ACINAR CELLS .1. MORPHOLOGICAL AND FUNCTIONAL CHARACTERIZATION [J].

MANGOS, JA ;

MCSHERRY, NR ;

BUTCHER, F ;

IRWIN, K ;

BARBER, T .

AMERICAN JOURNAL OF PHYSIOLOGY, 1975, 229 (03) :553-559

[5]

MANGOS JA, 1976, PEDIATR RES, V10, P357

[6]

Schneyer, 1967, SECRETORY MECHANISMS, P351

[7]

SCHNEYER L, 1967, HDB PHYSL 6, P497

[8] USE OF LIQUID SCINTILLATION SPECTROMETER FOR DETERMINING ADENOSINE TRIPHOSPHATE BY LUCIFERASE ENZYME [J].

STANLEY, PE ;

WILLIAMS, SG .

ANALYTICAL BIOCHEMISTRY, 1969, 29 (03) :381-&

← 1 →