ANTISERUM AGAINST RAT LUTEINIZING-HORMONE (LH) RECEPTORS

被引：17

作者：

LUBORSKY, JL ^{[1
]}

BEHRMAN, HR ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT PHARMACOL,NEW HAVEN,CT 06510

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)91192-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antiserum to soluble rat luteal LH receptors was produced in rabbits. The presence of LH receptor antibodies was shown by indirect immuno-precipitation of [125]hCG-soluble rat LH receptor-receptor antiserum complexes by antirabbit IgG. The antiserum bound LH receptors from rat ovaries and testes but not those from human or sheep ovaries. Unoccupied LH receptors competed with [125I]hCG occupied receptors in a dose-dependent fashion for antiserum binding. Extracts of rat brain and liver did not appear to bind to the antiserum. In addition, LH-dependent progesterone secretion from isolated rat ovarian cells was reduced by the antiserum although there was no change in hormone binding. © 1979.

引用

页码：1407 / 1413

页数：7

共 23 条

[1] INTERACTION OF MYASTHENIC SERUM GLOBULIN WITH ACETYLCHOLINE-RECEPTOR [J].